Coding

Part:BBa_K2155013:Design

Designed by: BingZhao Zhuo, Yue Yang   Group: iGEM16_NWPU   (2016-10-11)


BTT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 759
    Illegal NgoMIV site found at 1221
    Illegal AgeI site found at 223
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 130


Design Notes

Benzoylformate decarboxylase is flanked by prefix and suffix provided by iGEM.


Source

Pseudomonas putida CCUG 43661 and E.coli K-12

References